Identification and differentiation of three Nigerian Orseolia sp. (Orseolia nwanzei, Orseolia bonzii and Orseolia oryzivora) was carried out using Random Amplified Polymorphic DNA (RAPD) markers. Ninety operon primers were screened, from which 6 showed polymorphism among the three species tested, generating 54 bands, 69% of...
The genetic relationship and distance among rice varieties widely being used as parents by the African Rice Center (WARDA) are largely unknown but of great interest for breeding programs. The genetic relationship among 16 rice varieties was investigated using 83 EST and 174 SSR markers.
The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in...
Genetic mapping (also known as linkage mapping or meiotic mapping) refers to the determination of the relative position and distances between markers along chromosomes. Genetic map distances between two markers are defined as the...
Marker assisted backcrossing (MAB) is one of the most anticipated and frequently cited benefits of molecular markers as indirect selection tools in breeding programs. However, routine implementations of MAB in ongoing plant breeding...
The Africa Rice Center (WARDA) has developed several interspecific rice varieties by crossing the high yielding Asian rice (Oryza sativa subsp. japonica) with the locally adapted African rice (Oryza glaberrima). Eighteen varieties were named with the prefix NERICA (New Rice for Africa) but...
The very high resistance to Rice yellow mottle virus observed in the two rice varieties Gigante (Oryza sativa) and Tog 5681 (O. glaberrima) is monogenic and recessive. Bulked segregant analysis was carried out to identify AFLP markers linked to the resistance gene...
Rice yellow mottle virus (RYMV) accumulation in protoplasts and whole plants was investigated in two highly resistant cultivars, Tog5681 (Oryza glaberrima) and Gigante (Oryza sativa). Three susceptible cultivars, i.e. one O. glaberrima Tog5673 and...
Oryza glaberrima is an endemic African cultivated rice species. To provide a tool for evaluation and utilisation of the potential of O. glaberrima in rice breeding, we developed an interspecific O. glaberrima×Oryza sativa genetic linkage map. It was based on PCR markers...
Three cultivars of Oryza sativa (IR64, Azucena, and Gigante) and four cultivars of O. glaberrima (Tog5681, Tog5673, CG14, and SG329) were evaluated for their resistance to two isolates of rice yellow mottle virus (RYMV) by enzyme-linked immunosorbent assay (ELISA) and symptomatology...
